HiRise Scaffolding Report

January 19, 2021

Peregrine Phyllotis xanthopygus

Jay Storz

University of Nebraska

Contents

• Overview
• Contiguity Metrics
• BUSCO
• Pair Size Distribution
• Scaffolding Summary

Overview

Assembly	Total Length (bp)	N50	L50	N90	L90
Input Assembly	2,462,378,319	23,270,302	34	2,725,254	129
Dovetail HiRise Assembly	2,462,398,719	126,818,031	8	61,030,882	18

Contiguity Metrics

	Input Assembly	Dovetail HiRise Assembly
Largest scaffold	81,012,324	208,157,528
Number of scaffolds	2,453	2,250
Number of scaffolds > 1kbp	2,453	2,250
Number of gaps	0	184
Number of N's per 100 kbp	0.00	0.83

BUSCO

Assembly	Complete BUSCOs (C)	Complete and single-copy BUSCOs (S)	Complete and duplicated BUSCOs (D)	Fragmented BUSCOs (F)	Missing BUSCOs (M)	Total BUSCO groups searched
Input Assembly	252 (98.82%)	251 (98.43%)	1	1	2	255
Dovetail HiRise Assembly	252 (98.82%)	251 (98.43%)	1	0	3	255

• BUSCO version is: 4.0.5
• The lineage dataset is: eukaryota_odb10 (Creation date: 2020-09-10, number of species: 70, number of BUSCOs: 255)

Pair Size Distribution

HiRise Scaffolding Information