HiRise Scaffolding Report

January 19, 2021

Phyllotis xanthopygus

Jay Storz

University of Nebraska

Contents

• Overview
• Contiguity Metrics
• BUSCO
• Pair Size Distribution
• Scaffolding Summary

Overview

Assembly	Total Length (bp)	N50	L50	N90	L90
Input Assembly	2,660,359,323	25,285,049	33	4,829,996	119
Dovetail HiRise Assembly	2,660,372,323	147,873,771	8	59,266,063	19

Contiguity Metrics

	Input Assembly	Dovetail HiRise Assembly
Largest scaffold	134,922,640	206,300,460
Number of scaffolds	434	305
Number of scaffolds > 1kbp	434	305
Number of gaps	0	107
Number of N's per 100 kbp	0.00	0.49

BUSCO

Assembly	Complete BUSCOs (C)	Complete and single-copy BUSCOs (S)	Complete and duplicated BUSCOs (D)	Fragmented BUSCOs (F)	Missing BUSCOs (M)	Total BUSCO groups searched
Input Assembly	250 (98.04%)	249 (97.65%)	1	2	3	255
Dovetail HiRise Assembly	251 (98.43%)	250 (98.04%)	1	1	3	255

• BUSCO version is: 4.0.5
• The lineage dataset is: eukaryota_odb10 (Creation date: 2020-09-10, number of species: 70, number of BUSCOs: 255)

Pair Size Distribution

HiRise Scaffolding Information